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| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Plasma | 8 | 4.2% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Plasma | 3 | 6.6% |
| Sample type | Average % | Range |
|---|---|---|
| Serum | 87 | 82%- 92% |
| Hep Plasma | 116 | 113%- 120% |
| EDTA Plasma | 105 | 100%- 110% |
| Cit plasma | 101 | 89%- 107% |
Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat ACTH (adrenocorticotropic hormone) with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH’s primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
| Components | 1 x 96 tests |
|---|---|
| 10X Mouse/Rat ACTH (adrenocorticotropic hormone) Capture Antibody | 1 x 600µl |
| 10X Mouse/Rat ACTH (adrenocorticotropic hormone) Detector Antibody | 1 x 600µl |
| 10X Wash Buffer PT (ab206977) | 1 x 20ml |
| 5X Cell Extraction Buffer PTR (ab193970) | 1 x 1ml |
| Antibody Diluent 4BI | 1 x 6ml |
| Mouse/Rat ACTH (adrenocorticotropic hormone) Lyophilized Peptide | 2 vials |
| Plate Seals | 1 unit |
| Sample Diluent NS(ab193972) | 1 x 12ml |
| SimpleStep Pre-Coated 96-Well Microplate (ab206978) | 1 unit |
| Stop Solution | 1 x 12ml |
| TMB Development Solution | 1 x 12ml |
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