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ECM BIOSCIENCES/β-Adaptin (N-terminal region) Antibody/100 μl/AM2951

Adaptins are essential for the formation of adaptor complexes of clathrin-coded vesicles. Adaptins interact with the cytoplasmic domains of membrane-spanning receptors in the course of their endocytic/exocytic transport. They represent 1 of at least 4 components of intracellular transport and receptor downregulation pathways, the others being α/β/γ-adaptins, receptors, and ligand molecules. β-adaptins have a bipartite structure with invariant N-terminal and variable C-terminal domains. The former interact with the uniform component of coded vesicles, the clathrin lattice. In contrast, the C-terminal domain provides a set of recognition sequences for other proteins and therefore functions as part of a selector domain responsible for the specific entrapment of membrane-bound proteins in vesicles.

References

Sosa, M.A. (1996) Biocell. 20(3):301.

Boehm M. & Bonifacino J.S. (2002) Gene. 286(2):175.

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-β-Adaptin (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).

Clone (M295) was generated from a sequence corresponding to amino acids near the N-terminal region of the human β1 subunit of the adaptor related protein complex 1. This sequence has high homology to rat and mouse β1-Adaptin.

*For more information, see UniProt Accession Q10567

Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 106 kDa* protein corresponding to the molecular mass of β-Adaptin on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

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ECM BIOSCIENCES/β-Adaptin (N-terminal region) Antibody/100 μl/AM2951

References

*For more information, see UniProt Accession Q10567

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