Abcepta/Bax Antibody (BH3 Domain Specific)/400 µl/AP1302a

SPECIFICATION
CITATIONS: 13
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, FC, E
Primary Accession	Q07812
Other Accession	O02703, Q07814
Reactivity	Human, Mouse
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig
Calculated MW	21184 Da
Antigen Region	41-76 aa

Additional Information
Gene ID	581
Other Names	Apoptosis regulator BAX,Bcl-2-like protein 4,Bcl2-L-4,BAX,BCL2L4
Target/Specificity	This Bax antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-76 amino acids from human Bax.
Dilution	WB~~1:2000FC~~1:25
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Bax Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BAX
Synonyms	BCL2L4
Function	Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Cellular Location	[Isoform Alpha]: Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane [Isoform Gamma]: Cytoplasm.
Tissue Location	Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt"s lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines

Research Areas

Primary Antibodies
Crown Antibodies
Cancer
Cell Biology
Metabolism
Antibody Collections
Amyotrophic Lateral Sclerosis (ALS)
Viral process
Host-virus interaction
Apoptosis
Apoptosis signaling
Mitochondrion

Citations ( 0 )

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

Background

Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

References

Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003). Roucou, X., et al., J. Biol. Chem. 278(42):40877-40881 (2003). Cao, X., et al., Blood 102(7):2605-2614 (2003). McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003). Bidere, N., et al., J. Biol. Chem. 278(33):31401-31411 (2003).

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Abcepta/Bax Antibody (BH3 Domain Specific)/400 µl/AP1302a

Background

References