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| Concentration: | 1 mg / ml, determined by the Lowry method |
| Purification: | Repurified from HPLC |
| Purity: | ≥ 99% by SDS-PAGE |
| Buffer: | In 75 mM PBS, 75 mM NaCl, 0.02 % NaN3, 0.5 mM EDTA, pH 7.3. |
| Storage: | -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing. |
Human albumin constitutes around 55% of the protein present in the plasma of normal healthy individuals. It is a single polypeptide chain of 585 amino acids with a molecular weight of 66 kDa.
The functions of human serum albumin include maintaining oncotic pressure; binding substances or drugs; regulating endogenous substances, metabolism; plasma buffer; antioxidant function; maintaining microvascular integrity; anticoagulant effects; inflammatory/anti-inflammatory effects; cell-signaling process. (Nicholson et al., 2000; Evans, 2002) Albumin also has functions that may play a critical role and have a great impact on different types of diseases, including hypo-albumin aemia and peripheral oedema. (Caironi et al. 2009)
Caironi, Pietro, and Luciano Gattinoni. “The clinical use of albumin: the point of view of a specialist in intensive care.” Blood Tranfusion 7.4 (2009): 259-67.
Evans, T. W. "Review Article: Albumin as a Drug-biological Effects of Albumin Unrelated to Oncotic Pressure." Alimentary Pharmacology and Therapeutics 16 (2002): 6-11.
Nicholson, J. P., M. R. Wolmarans, and G. R. Park. "The Role of Albumin in Critical Illness." British Journal of Anaesthesia 85.4 (2000): 599-610.
| [P01] | 2003 | Weeks, B. L.; Camarero, J.; Noy, A.; Miller, A. E.; Stanker, L.; Yoreo, J. J. de (2003): A microcantilever-based pathogen detector. In Scanning 25 (6), pp. 297–299. DOI: 10.1002/sca.4950250605. |
| [P01] | 2001 | Wu, G.; Datar, R. H.; Hansen, K. M.; Thundat, T.; Cote, R. J.; Majumdar, A. (2001): Bioassay of prostate-specific antigen (PSA) using microcantilevers. In Nature Biotechnology 19 (9), pp. 856–860. DOI: 10.1038/nbt0901-856. |
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