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ACROBiosystems/Human Tyk2 Protein, His Tag (MALS verified)/50ug/TY2188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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ACROBiosystems/Human Tyk2 Protein, His Tag (MALS verified)/50ug/TY2

  • Synonym
    Tyk2,tyrosine kinase 2
  • Source
    Human Tyk2, His Tag (TY2-H5549) is expressed from Baculovirus-Insect cells. It contains AA Asn 575 - Leu 869 (Accession # P29597-1).
    Predicted N-terminus: Met
    Request for sequence
  • Molecular Characterization
    Online(Asn 575 - Leu 869) P29597-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 35.0 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 2 mM TCEP, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Human Tyk2, His Tag (Cat. No. TY2-H5549) SDS-PAGE gel

Human Tyk2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Tyk2, His Tag (Cat. No. ) MALS images

The purity of Human Tyk2, His Tag (Cat. No. TY2-H5549) was more than 90% and around 27-37 kDa verified by SEC-MALS.

Protocol
  • Background
    Tyk2 was the first member of the JAK family that was described (the other members are JAK1, JAK2, and JAK3). It has been implicated in IFN-α, IL-6, IL-10 and IL-12 signaling. Tyk2 functions primarily in IL-12 and type I-IFN signaling. Tyk2 deficiency has more dramatic effects in human cells than in mouse cells. However, in addition to IFN-α and -β and IL-12 signaling, Tyk2 has major effects on the transduction of IL-23, IL-10, and IL-6 signals. Recently, it has been recognized that IL-12 and IL-23 share ligand and receptor subunits that activate Tyk2. Tyk2 is activated by IL-10, and its deficiency affects the ability to generate and respond to IL-10.
  • References
    • (1)Colamonici O, et al. Mol Cell Biol. 1994. 14(12):8133-42.
    • (2)Velazquez L, et al. Cell. 1992. 70(2):313-22.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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