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AnshLabs/AMH (Canine) ELISA/96 well microtiter/AL-113

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Packaging

96 well microtiter

Detection

HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter

Dynamic Range

2, 0.28-18 ng/mL

Limit of Detection

0.055 ng/mL

Sample Size

20 µL

Sample Type

Serum

Assay Time

3.5 hours

Species Reactivity

Canine, Feline

Shelf Life

24 months

Availability

Worldwide

Anti-Müllerian hormone is a 140 kDa glycoprotein that is produced during normal embryogenesis by the Sertoli cells of the embryonic testis, causes the involution of the Müllerian duct, and inhibits female gonadogenesis by inducing apoptosis of target gonadal cells. It belongs to the transforming growth factor-β super family. AMH causes apoptosis of specific Anti-Müllerian inhibiting substance (MIS) receptor-bearing cells, while having no effect on cells without receptors. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary, and AMH inhibits recruitment of primordial follicles into the pool of growing follicles, and decreases responsiveness of growing follicles to FSH.

References:1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL52. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.

AMH (Canine) ELISA AL-116

Cusatti AC, Somoza R, Marchetti IA. Antimullerian Hormone (AMH) as biomarked of Granulosa Cell Tumor (GCT), case report. Abstract presented at the 20th European Veterinary Society for Small Animal Reproduction Congress, 29th June – 1st July 2017, Vienna Austria

All Products Cited Canine AMH ELISA, AL-116

Hollinshead FK, Walker C, Hanlon DW. Determination of the normal reference interval for anti-Müllerian hormone (AMH) in bitches and use of AMH as a potential predictor of litter size. Reprod Domest Anim. 2017 Apr;52 Suppl 2:35-40. PMID 27757997

All Products Cited Canine AMH ELISA AL-116

Kumar A, Kalra B, Patel A, Shah S. New Sensitive Anti-Müllerian Hormone (AMH) ELISAs for Non-Human Primate, Rodent, Equine, Bovine, Canine and Other Species. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.

All Products Cited Human/Primate AMH ELISA AL-105; Bovine AMH ELISA AL-114; Canine AMH ELISA AL-116; Equine and Ovine AMH ELISA AL-115; Rat and Mouse AMH ELISA AL-113

Themmen APN, Kalra B, Visser JA, Kumar A, Savjani G, Gier J, Jaques S. The use of anti-Müllerian hormone as diagnostic for gonadectomy status in dogs. Theriogenology 86 (2016) 1467–1474. PMID 27291082

All Products Cited AMH (Canine) ELISA AL-116; rec Hu AMH Ag AG-303-BA047

Yilmaz O, Toydemir T, Kirsan I, Ucmak Z, Karacam E. Anti-Mullerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches. Vet Res Commun. First published online June 24, 2015. PMID 26099303

All Products Cited Canine AMH ELISA AL-116

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  • MSDS
  • Scientific Poster
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