BD Biosciences/Recombinant Rat IL-2/5µg/555106

Description

Interleukin-2 (IL-2) is a potent, multifunctional cytokine produced by activated T lymphocytes. It is responsible for the activities attributed to T cell growth factor, thymocyte stimulation factor, thymocyte mitogenesis factor, killer helper factor, and T cell replacing factor. IL-2 stimulates the growth and differentiation of T cells, as well as cytokine production by T cells. In addition, IL-2 can contribute to the activation of monocytes/macrophages and NK and LAK cells and can augment B cell growth and differentiation.

Formulation and Purity

Recombinant rat IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/mlbiotechnology grade, low-endotoxin bovine serum albumin, glycerol and with no preservatives. The endotoxin level is ≤ 0.1 ng per μg of rat IL-2 protein, as measured in a LAL chromogenic assay. This recombinant rat IL-2 is a single band of approximately 16.5 kD as determined by SDS-PAGE analysis on 10 - 20% gels run under reducing conditions.

Preparation and Storage

This preparation contains no preservatives, thus it should be handled under aseptic conditions.Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at –80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein, such as human or bovine albumin, aliquoted and stored at ≤ -70°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 – 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 10 μg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.