产品资料

BD Biosciences/FITC Mouse Anti-Human Ig, κ Light Chain/20 µl/560950

Product DetailsRecommended AssayReferences

Description

The G20-193 monoclonal antibody specifically binds to human immunoglobulin light chain, kappa (κ). It does not react with human immunoglobulin λ light chains or human immunoglobulin heavy chains.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format

FormatFITC
Excitation SourceBlue 488 nm
Excitation Max494nm
Emission Max520nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Other Formats→

APC
APC-H7
Alexa Fluor® 700
BB515
BB700
BUV395
BUV496

BUV563
BUV615
BUV661
BUV737
BUV805
BV421
BV480

BV510
BV605
BV650
BV711
BV750
BV786
Biotin

PE
PE-CF594
PE-Cy™7
PerCP-Cy™5.5
Purified
R718
V450

Suggested Companion Products

FITC Mouse IgG1, κ Isotype Control RUO 100TestsCat No: 555748

PE Mouse Anti-Human CD19 HIB19RUO 100TestsCat No: 555413

Resources & Tools
Spectrum Viewer	Panel Designer	SpectrumViewer	Download TDS	Regulatory Document Website

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light.Do not freeze.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test.We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.