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AustralBiologicals/AUSTRAL Biologicals Catalog/1mg/PCA-2110a-9

AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human NFkappaB p50 IgG fraction polyclonal antibody

  • Catalog # PCA-2110a-9
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Catalog NumberPCA-2110a-9
Product IdPCA-2110a-9
Product NameRabbit anti-Human NFkappaB p50 IgG fraction polyclonal antibody
Temperature-20°C
Unit1mg
Price425
AvailabilityAvailable
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MAEDDPYLGRPE) corresponding to amino acids 1-12 of human NFkappaB p50. The IgG fraction was purified using Protein G-Sepharose.
IsotypeN/A
Mol WeightN/A
PurityN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts in ELISA and Western blot using the immunizing peptide (1: 500 dilution). Nuclear factor kappa light chain enhancer in B cells (NFkappaB) is a transcription regulator that consists of two subunits, 50 kDa DNA binding subunit (p50) and 65 kDa transactivation subunit (p65/Rel1A). p50 subunit has an isoform p52 and both proteins are derived from p105 precursor.The p50/p65 heterodimer remains in the cytosol in an inactive form bounded to its inhibitor IkappaB. Upon stimulation, phosphorylation of IkappaB releases NFkappaB complex which is traslocated into de nucleus and regulates the expression of genes that participate in immune, apoptotic, and oncogenic processes.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesLin, L. & Ghosh, S. (1996) Mol. Cell. Biol. 16, 2248-2254. Bassères, D.S. & Baldwin, A.S., (2006) Oncogene 25, 6017-6830.
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