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Biomedica/Big Endothelin-1 ELISA | BI-20082H/1 kit/BI-20082H

Assay Characteristics
  • Category number

    BI-20082H

  • Method

    Sandwich ELISA, HRP/TMB, 12×8-well detachable strips

  • Sample type

    Serum, EDTA plasma. citrate plasma, heparın plasma

  • Sample volume

    50 µl / well

  • Assay time

    4 h / 1 h / 30 min

  • Sensitivity

    0.02 pmol/l (= 0.086 pg/ml)

  • Standard range

    0 – 3 pmol/l (= 0 – 12.85 pg/ml)

  • Conversion factor

    1 pmol/l = 4.283 pg/ml (MW: 4.283 kDa)

  • Precision

    In-between-run (n=5): ≤ 5 % CV

    Within-run (n=10): ≤ 4 % CV

  • Specificity

    Endogenous and recombinant Big Endothelin-1 (Big ET-1 (1-38)).

  • Cross-reactivity

    Human ET1/2/3 (1-21): <1%, human ET2 (1-37): <1%, human ET1/2 (1-38): <1%, human BigET1/2 (22-38) : <1%, human BigET2 (22-37) : <1%,

    porcine BigET (1-39): 21%, rat BigET1 (1-39): 10%, Sarafotoxin: <1%

  • Use

    CE marked – for IVD use in the EU

  • Validation Data

    See validation data tab for: precision, accuracy, diltuion linearity, values for healthy donors, etc.

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