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Biomedica/Total Soluble Neuropilin-1 ELISA | BI-20409/1 kit/BI-20409188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Biomedica/Total Soluble Neuropilin-1 ELISA | BI-20409/1 kit/BI-20409

Assay Characteristics
  • Method

    Sandwich ELISA, HRP/TMB, 12×8-well detachable strips

  • Sample type

    Serum, EDTA plasma, citrate plasma, heparın plasma, urine, cell culture supernatant, human and non-human samples

  • Sample volume

    10 µl / well

  • Assay time

    30 min / 2 h / 1 h / 30 min

  • Sensitivity

    0.09 nmol/l (= 6.3 ng/ml)

  • Standard range

    0 – 12 nmol/l (= 0 – 836 ng/ml)

  • Conversion factor

    1 pmol/l = 69.7 pg/ml (MW: 69.7 kDa)

  • Specificity

    Endogenous and recombinant human soluble Neuropilin-1 (isoform 2 and 3).

  • Precision

    In-between (n=10): ≤ 10 % CV

    Within-run (n=6): ≤ 11 % CV

  • Cross-reactivity

    Due to the high sequence homology between human NPR1 and NPR1 of other species there is potential cross-reactivity with pig and rhesus macaque NPR1 and NPR1 of other species.

    Neuropilin-1 (NRP1) only. The amino acid sequence in the respective binding regions of the antibodies show no homology with NRP2. Thus, cross-reactivity to NRP2 is not expected.

  • Validation Data

    See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc

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