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Biomol/Cyclic GMP ELISA Kit/96 st-wells (96 strip wells)/Cay581021-96

Product information "Cyclic GMP ELISA Kit"
The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2" hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions. Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106 cells).
Keywords: cGMP EIA Kit, Guanosine 3",5"-cyclic mononucleotide, Guanosine 3",5"-cyclic monophosphate
Supplier: Cayman Chemical
Supplier-Nr: 581021-96

Properties

Application: ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H226, H301, H302, H311, H314, H315, H317, H318, H319, H332, H335, H351, H361
P Phrases: P201, P202, P210, P240, P241, P242, P243, P260, P261, P264, P270, P271, P272, P273, P280, P312, P321, P363, P301+P310, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P305+P351+P338, P308+P313, P332+P313, P333+P313, P337+P313, P361+P364, P362+P364, P370+P378, P403+P233, P403+P235
Caution Our products are for laboratory research use only: Not for administration to humans!
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