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5340:Recombinant Rat Interleukin-5 PRODUCT DESCRIPTION: The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. Interleukin-5 Rat Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13074 Dalton. The IL-5 is purified by proprietary chromatographic techniques.SOURCE: Escherichia Coli. FORMULATION: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. PURITY: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. STORAGE: Although stable at room temperature for 3 weeks, it should be stored desiccated below -18°C. Upon reconstitution store at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. SOLUBILITY: It is recommended to reconstitute in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. BIOLOGICAL ACTIVITY: The ED50 range=0.3-1.0 ng/ml as determined by the dose-dependant stimulation of the proliferation of BCL-1 cells. |
DevaTal, Inc, 644 Newkirk Ave, Hamilton NJ 08610 Phone: 609-586-1575, Fax: 609-586-6892 |
FOR RESEARCH USE ONLY |
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