Format
96-WellPlate
SpeciesReactivity
Homosapiens(Human)
SampleType
Serum,Plasma,Celllysatesandother
BIOLOGicalfluids.
DetectionRange
3.12-200pg/mL
Sensitivity
1.42pg/mL
EstimatedTurnaroundTime
7-11businessdays
TargetName
ClusterOfDifferentiation83
TargetSynonyms
BL11,HB15,ActivatedBLymphocytes,Immunoglobulin,B-cellactivationprotein,CellsurfaceproteinHB15
Precision
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelClusterOfDifferentiation83weretested20timesononeplate,respectively.Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelClusterOfDifferentiation83weretestedon3differentplates,8replicatesineachplate.CV(%)=SD/meanX100;Intra-Assay:CV<10%;inter-assay:><>
DetectionMethod
Colorimetric
AssayTime
3hours
Application
AssayType
Double-antibodySandwich
ShippingCondition
Icepacks
Storage
Shortterm:4°C;Longterm:seemanual.
PrecautionofUse
TheStopSolutionisacidic.Donotallowtocontactskinoreyes.
ExpiryDate
8months
UniProtID
Q01151
Specificity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofClusterOfDifferentiation83
CrossActivity
Nosignificantcross-reactivityorinterferencebetweenClusterOfDifferentiation83andanalogueswasobserved.
AssayProcedure
1.Prepareallreagents,samplesandstandards;2.Add100µLstandardorsampletoeachwell.Incubate2hoursat37°C;3.Aspirateandadd100µLpreparedDetectionReagentA.Incubate1hourat37°C;4.Aspirateandwash3times;5.Add100µLpreparedDetectionReagentB.Incubate30minutesat37°C;6.Aspirateandwash5times;7.Add90µLSubstrateSolution.Incubate10-20minutesat37°C;8.Add50µLStopSolution.Readat450nmimmediately.
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
Restrictions
ForResearchUseOnly.Notforuseinvitrodiagnosticprocedures,druguse,orforadmi
NISTrationtohumansoranimals.
QualitySystems
ThekitismanufacturedatISO9001andISO13485certifiedfacilities.
QualityAssurance
BiomatikELISAkitsarenottypicallypre-assembledasfinishedproductsduetolimitedshelflife.Beforeshipping,eachkitisassembledandtestedtoensurethatitmeetsspecificationsbeforeshipping.MinorchangesmayoccurtotheDetectionRange,Sensitivity,andPrecision.AllkitsaretestedtoconfirmthattheyfallwithintheirdefinedInter-andIntra-assaycoefficientofvariation.PleasealwaysrefertothehardcopymanualincludedinThekitbeforeexperiment.
ResearchArea
CD&Adhesionmolecule,Infectionimmunity,Immunemolecule,Dermatology
