EnCorBio/Mouse Monoclonal Antibody to Aldolase C Cat# MCA-4A9/50µL of 1mg/mL/MCA-4A9188bio精品生物—专注于实验室精品爆款的电商平台

Mouse Monoclonal Antibody to Aldolase C Cat# MCA-4A9

$120.00 – $800.00

Aldolases are important glycolytic cytosolic enzymes which catalyse the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate. There are three aldolase isozymes coded by three distinct genes in mammals, namely aldolases A, B, and C. Aldolase A is heavily expressed in muscle enzyme and aldolase B is heavily expressed in liver-specific enzyme (1-5). In the adult, aldolase C is the brain-specific isozyme expressed in astrocytes and a few classes of neurons, notably Purkinje cells (4). Appropriate antibodies to aldolase C are therefore useful to identify astrocytes in cell culture and sections, and the enzyme may be over expressed in some forms of cancer (6). Recent studies also suggest that detection of aldolase C in blood may be a useful marker of the severity of traumatic brain injury (7).The MCA-4A9 antibody was made against a recombinant construct including the N-terminal peptide of human aldolase C. Later mapping studies localized the epitope to within the peptide HSYPALSAEQKKELSDIA, amino acids 3-20. Since the three aldolase enzymes are quite similar in amino acid sequence many available antibodies to one protein have usually undocumented cross-reactivity with the other two. However we have used appropriate recombinant constructs to shown that MCA-4A9 is completely specific for aldolase C with no reactivity with either aldolase A or B. Since we know the identity of the peptide used to generate MCA-4A9 is firmly mapped to the N-terminus of aldolase C, a region in which there is considerable variability between the three aldolase enzymes. Mouse select image at left for larger view.

SKU: mca-4a9Categories: Cell Type Marker, Cytoplasmic Enzyme, Epitope Mapped Antibodies, Mouse Monoclonal Antibodies

Overview
Additional Info
References
Data Sheets

Name:	Mouse Monoclonal Antibody to Aldolase C: N-terminus specific
Immunogen:	N-terminal sequence MPHSYPALSAEQKKELSDIA
HGNC Name:	ALDOC
UniProt:	P09972
Molecular Weight:	40kDa
Host:	Mouse
Isotype:	IgG1
Species Cross-Reactivity:	Human, rat, mouse, cow, pig
RRID:	AB_2571880
Format:	Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃
Applications:	WB, IIF/ICC, IHC
Recommended Dilutions:	WB: 1:2,000. IF/ICC or IHC: 1:1,000.
Storage:	Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.

Additional Data

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldolaseC, MCA-4A9, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, CPCA-MAP2, dilution 1:10,000 in red. The blue is Hoechst staining of nuclear DNA. The aldolase C antibody labels cytosolic protein expressed in the glial cells, while theMAP2 antibody stains dendrites and perikarya of mature neurons.

1. Popovici T, et al. Localization of aldolase C mRNA in brain cells. FEBS Lett. 268:189-193 (1990).2. Weber A, et al. Dietary Control of Aldolase B and L-type Pyruvate Kinase RNAs in Rat. J. Biol. Chem. 259:1798-802 (1984).3. Mukai T, et al. The structure of the brain-specific rat aldolase C gene and its regional expression. BBRC 174:1035-42 (1991).4. Royds J, et al. Monoclonal antibody to aldolase C: a selective marker for Purkinje cells in the human cerebellum. Neuropathol. Appl. Neurobiol. 13:11-21 (1987).5. Thompson R., Kynoch P. Willson V. Cellular localization of aldolase C subunits in human brain. Brain Res. 232:489-93 (1982).6. Schapira F, Reuber M, Hatzfeld A. Resurgence of two fetal-type of aldolases (A and C) in some fast-growing hepatomas. BBRC 40:321-27 (1970).7. Halford J, et al. New astroglial injury-defined biomarkers for neurotrauma assessment. J. Cereb. Blood Flow Metab. 37:3278-99 (2017).

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EnCorBio/Mouse Monoclonal Antibody to Aldolase C Cat# MCA-4A9/50µL of 1mg/mL/MCA-4A9

Mouse Monoclonal Antibody to Aldolase C Cat# MCA-4A9