EnCorBio/Mouse Monoclonal Antibody to TDP43 Cat# MCA-3H8/50µL of 1mg/mL/MCA-3H8188bio精品生物—专注于实验室精品爆款的电商平台

Mouse Monoclonal Antibody to TDP43 Cat# MCA-3H8

$120.00 – $800.00

TDP43 was originally identified as a protein which binds to the “transactivation response” (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (1). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain a two central copies of the ~90 amino acid RNA recognition motif or RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. It also has a single bipartite nuclear localization sequence in the N-terminus region in front of the first RRM domain, and the C-terminal region, beyond the second RRM domain is rich in glycine, asparagine and glutamine. TDP43 is normally expressed in the mammalian CNS but accumulates in nuclear inclusions in the neurons of patients with frontotemporal lobar degeneration (FTLD), amyotrophic lateral sclerosis (ALS), Alzheimer’s disease and other pathologies (2-4). Disease related point mutations in TDP43 are almost always found in the C-terminal region. TDP43 is related in protein sequence to FUS/TLS, EWSR1 and TAF15 and all four proteins have a tendency, especially when mutated, to aggregate into inclusions and are implicated in a variety of neurological disorders (5,6).The MCA-3H8 antibody was made against full length recombinant human protein and works well on western blots and on histological sections, staining FTLD and ALS related inclusion bodies, see “Additional Info” tab. It has also been shown to identify inclusions seen in Alzheimer’s disease (4). We have shown that MCA-3H8 reacts with TDP43 from human, cow, pig, mouse, rat and other mammals and that the epitope is in the center the peptide HNSNRQLERSGRFGGNPGGF, amino acids 264-283, C-terminal to second RRM domain of the human sequence. This is the region typically in TDP43 inclusions (see here). This peptide is 100% conserved in a wide variety of mammalian species so it is likely that the antibody is effective on many other species also. The KD for this antibody in 1.16 X 10^-9M. The antibody is also an excellent marker of nuclei in cell fractionation experiments. Mouse select image at left for rather view, and on “additional info” tab for more information.

SKU: mca-3h8Categories: Cell Type Marker, Epitope Mapped Antibodies, Kinetics, Mouse Monoclonal Antibodies, Pathology Related MarkerTag: Active

Overview
Background
Additional Info
References
Data Sheets

Name:	Mouse monoclonal antibody to TDP43
Immunogen:	Full length recombinant human TDP43 expressed in and purified from E. coli.
HGNC Name:	TARDBP
UniProt:	Q13148
Molecular Weight:	43kDa
Host:	Mouse
Isotype:	IgG1
Species Cross-Reactivity:	Human, rat, mouse, cow, pig, horse
RRID:	AB_2572387
Format:	Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃
Applications:	WB, IF/ICC, IHC
Recommended Dilutions:	WB: 1:5,000. ICC/IF and IHC 1:1,000
Storage:	Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.

TDP43 was originally identified as a protein which binds to the “transactivation response” (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (1). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain a two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus.The protein is frequently referred to by the acronym TDP43 corresponds to “TAR DNA binding protein of molecular weight 43 kDa”. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (ALS, 2). The protein is present in these inclusions in a partially degraded, hyperphosphorylated and ubiquitinated form. For a recent review of the role in TDP43 in various neurological disorders see reference 3.This antibody has been used in a recent peer reviewed publication, showing that it works well on western blots and on histological sections (4). TDP43 is one of the RNA binding proteins which appear to be aggregation prone particularly when mutated, these aggregates being implicated in a variety of neurodegenerative diseases (5,6). Further images of histological staining using this antibody showing clears staining of ALS related inclusion bodies is shown here. MCA-3H8 is a mouse IgG1 class antibody and was raised against full length recombinant human TDP43 expressed in and purified from E. coli. MCA-3H8 is known to react with TDP43 from human, cow, pig, mouse, rat and other mammals. The epitope for this antibody is within the peptide HNSNRQLERSGRFGGNPGGF, amino acids 264-283 in the C-terminal region of the human sequence. This peptide is 100% conserved in a wide variety of mammalian species so it is likely that the antibody is effective on many other species also.

The mouse monoclonal antibody to TDP43, MCA-3H8, is a robust marker of TDP43 positive inclusions in ethanol fixed and paraffin embedded sections, as shown here on a section of human brain from a patient with fronto-temporal dementia (FTLD). Image courtesy of Dr. Benoit Giasson of the Center for Translational Research in Neurodegenerative Disease (CTRND) at the University of Florida. Mouse select image for a larger view.

1. Ou SH. et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J Virol. 69:3584-96 (1995).2. Neumann M. et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314:130-33 (2006).3. Forman MS, Trojanowski JQ and Lee VM-Y. TDP-43: a novel neurodegenerative proteinopathy. Curr. Opin. Neurobiol. 17:548-55 (2007).4. Wilson AC, Dugger BN, Dickson DW and Wang DS. TDP-43 in aging and Alzheimer’s disease – a review. Int. J. Clin. Exp. Pathol. 4:147-55 (2011).5. Da Cruz S, Cleveland DW. Understanding the role of TDP-43 and FUS/TLS in ALS and beyond. Curr. Opin. Neurobiol. 21:904-19 (2011).6. Couthouis J, et al. A yeast functional screen predicts new candidate ALS disease genes. PNAS 108:20881-90 (2011).

The antibody has also been sold through many OEM partners, and peer-reviewed publications making use of it can be found by searching Google Scholar for “3H8 AND TDP43 AND antibody” or simply by selecting this link.

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EnCorBio/Mouse Monoclonal Antibody to TDP43 Cat# MCA-3H8/50µL of 1mg/mL/MCA-3H8

Mouse Monoclonal Antibody to TDP43 Cat# MCA-3H8