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Catalog Number:
E011056-1Amount:
50μg/50μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PTEN (phospho-Ser380/Thr382/Thr383) antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382/383.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC:1:50~100 IF:1:100~1:200Swiss-Prot No.:
P60484References:
Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.
P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Western blot analysis of extract from HT29 cells untreated or treated with calyculin A, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Price:
145$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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