Author: Parul Mehrotra, Najmuddin Saquib and Kanury V. S. Rao, Date: 2015-04-20 [Abstract] Fluctuations in metabolite levels in mammalian cells are the most direct form of readout of the cellular metabolic state. The current protocol describes a method for pulse labeling and subsequent isolation of metabolites from adherent mammalian cells. The isolated metabolites can be identified and quantified by mass-spectrometry, allowing for estimation of the rates of synthesis and removal of metabolites from the system being analyzed. [摘要] 哺乳动物细胞中代谢物水平的波动是细胞代谢状态的最直接形式的读出。 当前的协议描述了用于脉冲标记和随后从贴壁哺乳动物细胞分离代谢物的方法。 分离的代谢物可以通过质谱法鉴定和定量，允许估计代谢物从所分析的系统的合成速率和去除速率。 公司名称: Euriso-Top 产品编号: CLM-481 Similar Protocol Determining Efficiency and Selectivity of Lipid Extraction by Perturbing Agents from Model ...同位素, CLM-481, Euriso-TopD-Glucose, (6-13C, 99%) D-葡萄糖（6-13C，99％）D
