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Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.
Additionally, we also offer 2-color beads in the color combinations red/orange, red/green and red/blue.
On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.
Fields of Application:
Advantages:
3D stack of GATTA-Beads R measured at VUmc, Amsterdam, on a Leica TCS SP8 STED 3X. 3D PSF distillation and rendering by Scientific Volume Imaging using the MIP renderer in Huygens Professional
product properties of GATTA-Beads| Bead R | Bead O | Bead G | Bead B | |
|---|---|---|---|---|
| Color | red | orange | green | blue |
| Available fluorophores | ATTO647N | ATTO 594 | ATTO 542 | Oregon Green 488 |
| Alternative fluorophores | Abberior STAR 635P | - | Cy3 or Alexa Fluor 555 | Alexa Fluor 488 or ATTO 490LS |
| Recommended laser source | 630-650 nm | 565-610 nm | 515-540 nm | 480-505 nm |
| Diameter | 23 nm | 23 nm | 23 nm | 23 nm |
| Surface density | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² |
| Price (sample on glass slide) | 300,-€ | 300,-€ | 300,-€ | 300,-€ |
| Price (sample in solution) | 500,-€ | 500,-€ | 500,-€ | 500,-€ |
| Bead RO | Bead RG | Bead RB | |
|---|---|---|---|
| Color | red / orange | red / green | red / blue |
| Available fluorophores | ATTO647N / ATTO594 | ATTO647N / ATTO542 | ATTO647N / Oregon Green 488 |
| Recommended laser source | 630-650 nm, 565-610 nm | 630-650 nm, 515-540 nm | 630-650 nm, 480-505 nm |
| Diameter | 23 nm | 23 nm | 23 nm |
| Surface density | ≈ 1/µm² | ≈ 1/µm² | ≈ 1/µm² |
| Price (sample on glass slide) | 400,-€ | 400,-€ | 400,-€ |
| Price (sample in solution) | 600,-€ | 600,-€ | 600,-€ |
| Fluorophore | Our notation | Excitation / nm | Emission / nm |
| ATTO 647N | R | 647 | 664 |
| Abberior Star 635P | R | 635 | 651 |
| ATTO 594 | O | 594 | 626 |
| ATTO 542 | G | 542 | 562 |
| Cy3 | G | 554 | 568 |
| Alexa Fluor 555 | G | 555 | 580 |
| Oregon Green 488 | B | 488 | 526 |
| Alexa Fluor 488 | B | 488 | 525 |
| ATTO 490LS | B | 490 | 658 |
Download GATTA-Beads product sheet
Full size super-resolution Image of GATTA-Bead R
Full size super-resolution Image of GATTA-Bead G
Full size super-resolution Image of GATTA-Bead B
J.J. Schmied, R. Dijkstra, M. Scheible, G. M. R. De Luca, J. J. Sieber, GATTAquant GmbH, Scientific Volume Imaging (SVI), Leica Microsystems (2016): Measuring the 3D STED PSF with a new Type of Fluorescent Beads – Science Lab
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