产品资料

GATTAquant/GATTA-Beads/ATTO647N / ATTO594

GATTA-Beads

gattabeads.com_link.png

Small, bright and cutting-edge. Experience the highest brightness density in the world.

Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.

Additionally, we also offer 2-color beads in the color combinations red/orange, red/green and red/blue.

On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.

Fields of Application:

Setup calibration
2D STED measurements
3D STED PSF distillation and deconvolution
Tracking and drift-correction

Advantages:

Size: d = 23 nm
Brightness: the highest brightness density
Homogeneity: super small size and intensity distribution
Flexibility: change color or add chemical groups

3D stack of GATTA-Beads R measured at VUmc, Amsterdam, on a Leica TCS SP8 STED 3X. 3D PSF distillation and rendering by Scientific Volume Imaging using the MIP renderer in Huygens Professional

product properties of GATTA-Beads

	Bead R	Bead O	Bead G	Bead B
Color	red	orange	green	blue
Available fluorophores	ATTO647N	ATTO 594	ATTO 542	Oregon Green 488
Alternative fluorophores	Abberior STAR 635P	-	Cy3 or Alexa Fluor 555	Alexa Fluor 488 or ATTO 490LS
Recommended laser source	630-650 nm	565-610 nm	515-540 nm	480-505 nm
Diameter	23 nm	23 nm	23 nm	23 nm
Surface density	≈ 1/µm²	≈ 1/µm²	≈ 1/µm²	≈ 1/µm²
Price (sample on glass slide)	300,-€	300,-€	300,-€	300,-€

Price (sample in solution)	500,-€	500,-€	500,-€	500,-€

	Bead RO	Bead RG	Bead RB
Color	red / orange	red / green	red / blue
Available fluorophores	ATTO647N / ATTO594	ATTO647N / ATTO542	ATTO647N / Oregon Green 488
Recommended laser source	630-650 nm, 565-610 nm	630-650 nm, 515-540 nm	630-650 nm, 480-505 nm
Diameter	23 nm	23 nm	23 nm
Surface density	≈ 1/µm²	≈ 1/µm²	≈ 1/µm²
Price (sample on glass slide)	400,-€	400,-€	400,-€

Price (sample in solution)	600,-€	600,-€	600,-€

fluorophore properties

Fluorophore	Our notation	Excitation / nm	Emission / nm
ATTO 647N	R	647	664
Abberior Star 635P	R	635	651
ATTO 594	O	594	626
ATTO 542	G	542	562
Cy3	G	554	568
Alexa Fluor 555	G	555	580
Oregon Green 488	B	488	526
Alexa Fluor 488	B	488	525
ATTO 490LS	B	490	658

product sheet

Download GATTA-Beads product sheet

Full size super-resolution Images

Full size super-resolution Image of GATTA-Bead R

Full size super-resolution Image of GATTA-Bead G

Full size super-resolution Image of GATTA-Bead B

Publications

J.J. Schmied, R. Dijkstra, M. Scheible, G. M. R. De Luca, J. J. Sieber, GATTAquant GmbH, Scientific Volume Imaging (SVI), Leica Microsystems (2016): Measuring the 3D STED PSF with a new Type of Fluorescent Beads – Science Lab

References / Experience Reports

Leica Microsystems SVI Abberior Instruments.png

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒