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ATTO514-Actinα-actin from skeletal muscle, rabbit
Cat. #: 8154-02
.................................................................................................................................................................................................................................................................................................................................................................................................................ApplicationsActin assays for epifluorescence and high resolution microscopy, FRET, biochemical actin assays.
Description
Protein | ATTO514-Actin |
Origin | Skeletal muscle alpha actin, rabbit |
Molecular mass | 42kDa |
Protein description | Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids.ATTO514-Actin is chemically modified G-actin with an ATTO514 fluorescent group covalently bound to lysine residues.ATTO514-Actin (λex511nm, λem533nm)possesses the polymerization properties of native G-actin. |
Conjugation ratio | This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. The precise DOL is stated in the DataSheet supplied with the product. |
Properties
Form | Lyophilized, ready-to-use. |
Quantityper unit | 5x100 µg |
Buffer | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution. |
Purity | >99%by scanning densitometry. |
Purification notes | Purified by GPC from rabbit skeletal muscle. |
Protein concentration | Determined by the Biuret method. |
Storage instructions | ATTO514-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. SolubilizedATTO-Actinis kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
Material and Safety Data Sheet
References
Kank2activatestalin,reducesforcetransductionacrossintegrinsandinducescentraladhesionformation.
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.
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