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ATTO514-Actin for TIRFMNon-muscle actin from brain, porcine
Cat. #: 8354-03
..........................................................................................................................................................................................................................................................................................................................................................................................................................................................Description
Protein | ATTO514-Actin |
Origin | Non-muscle β/γfrom brain, porcine |
Molecular mass | 42kDa |
Protein description | Actin from rnon-miscle sources are a mixture ofβ/γnon-muscle actins. These isoforms are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids.ATTO514-Actin is chemically modified G-actin with an ATTO514 fluorescent group covalently bound to lysine residues.ATTO514-Actin (λex511nm, λem533nm)possesses the polymerization properties of native G-actin. |
Conjugation ratio | This product is supplied with a degree of labelling (DOL) of ~ 0.3 and is thus ready-to-use for TIRFM and other high-or super-resolution imaging techniques.. |
Properties
Form | Lyophilized, ready-to-use. |
Quantityper unit | 2x50µg |
Buffer | 2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution. |
Purity | >99%by scanning densitometry. |
Purification notes | Purified by GPC from rabbit skeletal muscle. |
Protein concentration | Determined by the Biuret method. |
Storage instructions | ATTO514-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. SolubilizedATTO-Actinis kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
Material and Safety Data Sheet
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