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GST-VCAHuman recombinant WAVE VCA-domain, GST-taggedCat. #: 8416-01
Description
| |
Protein | GST-VCA |
Origin | recombinant, human |
Molecular mass | ~37.5kDa |
Protein description | TheVCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASPfamily verprolin homologous) activatein vitrothe nucleation ability ofArp 2/3, which is itself a constitutively inactive complex.The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration. |
Actin interaction | Activator of theArp 2/3 protein complex(Cat.#: 8413-01). |
Properties
| |
Form | Lyophilized, ready-to-use. |
Quantity per unit | 1x250 µg |
Buffer | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 250 µlultrapure water to obtain a 1.0 mg/ml solution. |
Purity | >99%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration. The protein can detected with anti-GST antibodies using suited immunological detection systems. |
Protein concentration | Determined at OD280 (0.1%=1.25) |
Storage instructions | GST-VCA isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Structures on RCSB (WAVE)InitiationofDNAreplicationrequiresactindynamicsandforminactivity.Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.
Structure and control of the actin regulatory WAVE complex.
Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK.
Nature. 2010 Nov 25;468(7323):533-8. doi: 10.1038/nature09623.
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