Fireflyluciferaseiswidelyusedasareportergeneforstudyinggeneregulationandfunction,andforpharmaceuticalscreening(1).Itisaverysensitivegeneticreporterduetothelackofanyendogenousluciferaseactivityinmammaliancellsortissuesandtheverynatureofbioluminescence(2).Fireflyluciferaseisa62,000daltonproteinwhichisactiveasamonomeranddoesnotrequiresubsequentprocessingforitsactivity.TheenzymecatalyzesATP-dependentD-luciferinoxidationbyoxygenintooxyluciferinwithemissionoflightcenteredon560nm.Aswithmanyenzymes,fireflyluciferasefollowsMichaelis-Mentenkinetics,andasaresultmaximumlightoutputisnotachieveduntilthesubstratesandco-factorarepresentinlargeexcess.Whenassayedundertheseconditions,lightemittedfromthereactionisdirectlyproportionaltothenumberofluciferaseenzymemolecules.TheFireflyLuciferaseAssayKitisdesignedfordetectionandquantificationoffireflyluciferasereporterenzymefromculturedcellsinasimple,efficientandlinearfashion.
FireflyLuciferaseAssayKit(Lyophilized)performscomparablytoBiotium’sFireflyLuciferaseAssayKit2.0,butincludesassaybufferinlyophilizedformatformoreeconomicalshippingandconvenientstorageat-20°C.
KitComponents:
Features:
MoreLuciferaseKits
Catalognumber | Product | Description |
---|---|---|
30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30028 | Steady-LucFireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
30020 | ATP-GloBioluminometricCellViABIlityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
1.Anal.Biochem.219:169-181(1994).
2.Anal.Biochem.175:5-13(1988).
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn