Cell Line Information:
Designations: PC-12
Organism: Rattus norvegicus (rat)
Tissue: pheochromocytoma; adrenal gland
Gender: male
Morphology: small irregularly shaped cells
Tumorigenic: yes, in New England Deaconess Hospital strain rats
Karyotype: 40 chromosomes; 38 autosomes plus XY
Products: catecholamines; dopamine; norepinephrine
Receptors expressed: nerve growth factor (NGF)
Growth Properties: floating clusters; few scattered lightly attached cells.
Depositor: B. Patterson
Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. The cells do not synthesize epinephrine.
The Transfection Reagent:
Transfection of suspension cells, or suspension/adherent mixed cells, such as PC-12 Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
PC-12 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting PC-12 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PC-12 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
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PC-12 Cell Avalanche™ Transfection Reagent (rat pheochromocytoma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell |
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