WesternLumaxLight SuperiorHRP substrate

The best choice for routine, film-imaged Western blots WesternLumaxLight Superior HRP substrate is more

sensitive than other chemiluminescent substrates, soyou can conduct shorter exposures, load less sample,

or use up to ten-times less antibody. Save time and money with WesternBright ECL.

DescriptionWesternLumaxLight Superior is a horseradish peroxidase substrate optimized for chemiluminescent Westernblots imaged using X-ray film. WesternLumaxLightECLrequiresaslittle as ten times less antibody thanother substrates,allowing you to save precious antibodies and samples. Additionally, the WesternLumaxLightsignal is long lasting,allowing multiple exposures to be carried out without substantial signal decay.

WesternLumaxLight Superior detects equal amounts of protein with up to ten times less antibody.

Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions

shown,and detected with either WesternLumaxLight Superior or Pierce ECL (Thermo Scientific) accor

-ding to themanufacturers’instructions. The blots were imaged simultaneouslyon the same piece

of film.WesternLumaxLight Superior produced thesame signal with ten times less primary and five

times lesssecondary antibody.

Customerfeedback

AftertryingWesternLumaxLight Superior,weimmediatelyswitchedfromourusualbrandandnowuseitonaweeklybasis.

Itproducesastrong,long-lastingsignalwithlowbackgroundatamuchlowercost.

AssistantProfessor,BrighamYoungUniversity

WesternLumaxLight Superiorisbetterthan(theECLsubstrate)Iusedtousebecauseofitshighsensitivity.Most of my

researchinvolvesdetectinglowamountsofinducedproteins,suchasgp91phoxandHIF-1. Ihad some challenge to

detecttheseproteinswhenIusedothersubstrates;however,WesternLumaxLight Superiorhelpstodetectthesetargets.

Now,IcandetecttheseproteinsusinglesssamplewiththeWesternLumaxLight Superiorsystem.Mywholelabhas now

switchedtoWesternLumaxLight Superior.

SeniorResearchLabSpecialist,UniversityofMichigan

WehaveswitchedtotheWesternLumaxLight Superiorreagentforstraightwesternandco-IPwesternblotting. We

haveusedlessantibodiestosuitourvariousneedseasily.Bytheway,theWesternBrightreagentalsoworkedbeaut

-ifullyduringarecentdemoofacompetitor’schemiluminescentimager!Itperformedbetterthanthevendor‘sown

proprietaryreagentmadeespeciallyforthatmachine!

LabManager,UniversityofNorthCarolinaatChapelHill