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Description | alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively. |
Catalog Number | IW-MA1107 |
Quantity | 9 ml |
Host | Mouse |
Clone | Tub-1 |
Isotype | Mouse IgG1 |
Immunogen | Microtubules from chicken embryo brain. |
Purity | Purified by the goat anti-mouse IgG affinity chromatography. |
Conjugate | Unconjugated |
Species Reactivity | Human, rat. Not tested in other species. |
Positive Control | Brain |
Cellular Localization | Cytoplasmic |
Form | Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. |
Storage | Store at 2-8 °C. Do not freeze. |
Applications | IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Not tested. ICC: Not tested. |
Limitations | This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. |
Precautions | Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. |
References | 1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983. 2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985. |
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