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indoor biotechnologies/LoTox™ Natural Ara h 6 (LTN-AH6-4)/1mg/LTN-AH6-4

Allergen:nAra h 6 (Arachis hypogaea allergen 6)
Unit:1mg
Availability:10 Business Day Lead Time
Source: Light roasted peanut flour (Runner cultivar)
Mol. Wt:14.5 kD
Purification:

From delipidated peanut extract by multi-step chromatography.Purity on silver stained SDS-PAGE >95%.

Concentration:

See product insert.

Formulation:

Preservative and carrier-free in sterile, endotoxin-free,phosphate buffered saline, pH 7.4

Storage:Store at -20ºC
Notes:

(1) Trace level of Ara h 2 is 0.01%

(2) Ara h 1 undetectable

(3) A LoTox™ product, Endotoxin < 0.03 EU/µg.

(4) Avoid repeated Freeze/Thaw cycles.

PDB:
Product Resources:LoTox™ Natural Ara h 6 Certificate of Analysis
Allergens are provided for research and commercial use in vitro:Not for human in vivo or therapeutic use.
References:
  1. Kleber-Janke T, Crameri R, Appenzeller U, Schlaak M, Becker WM. Selective cloning of peanut allergens, including profilin and 2S albumins, by phage display technology. Int Arch Allergy Immunol 1999; 119: 265-74.
  2. Suhr M, Wicklein D, Lepp U, Becker W. Isolation and characterization of natural Ara h 6: Evidence for a further peanut allergen with putative clinical relevance based on resistance to pepsin digestion and heat. Mol Nutr Food Res 2004; 48: 390-9.
  3. Koppelman S, de Jong G, Laaper-Ertmann M, Peeters K, Knulst A, Hefle S, Knol E. Purification and immunoglobulin E-binding properties of peanut allergen Ara h 6: evidence for cross-reactivity with Ara h 2. Clin Exp Allergy 2005; 35: 490-7.
  4. Porterfield HS, Murray KS, Schlichting DG, Chen X, Hansen KC, Duncan MW, Dreskin SC. Effector activity of peanut allergens: critical role for Ara h 2, Ara h 6, and their variants. Clin Exp Allergy 2009; 39: 1099-108.
  5. Blanc F, Adel-Patient K, Drumare M, E, Wal J, Bernard H. Capacity of purified peanut allergens to induce degranulation in a functional in vitro assay: Ara h 2 and Ara h 6 are the most efficient elicitors. Clin Exp Allergy 2009; 39: 1277-85.
  6. Flinterman AE, Pasmans S, den Hartog Jager CF, Hoekstra MO, Bruijnzeel-Koomen C, Knol EF, van Hoffen E. T cell responses to major peanut allergens in children with and without peanut allergy. Clin Exp Allergy 2010; 40: 590-7.
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