Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
ImmunoChem/Acetyl Lysine Antibody, Agarose -2mg/2 mg/ICP0388-2MG188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

ImmunoChem/Acetyl Lysine Antibody, Agarose -2mg/2 mg/ICP0388-2MG

Acetyl Lysine Antibody, Agarose -2mg

Catalog # Pack Size Price(USD)
ICP0388-2MG 2 mg $550.00

Quantity:

Product Description

The acetylated lysine antibody is immobilized to agarose beads via amide linkages. The product can be utilized as an affinity matrix for rapid isolation and purification of proteins/peptides with acetyl lysine residues.

The acetylated peptides eluted from acetyl-lysine-specific affinity chromatography (ICP0388).
Approximately 5 mg of trypsinated crude protein from TSA treated MMRU cells wereloaded.
The maximum binding of acetylated BSA with ICP0388-2MG.
-50 µl of ICP0388-2MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min.
- After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl
-5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE and blotted wih monoclonal Mouse anti-Acetylated Lysine (ICP0390)
Formulation

0.5 mL of agarose beads suspended in 1 mL of slurry

Antibody Immobilized

4 mg/ml antibody is covalently linked through amide bonds with NHS activated-SMCC, which further conjugates to thiolated agarose beads via thiol ether bonds.

Specificity

The antibody selectively captures proteins/peptides wtih acetylated lysine residues (N-epsilon). No cross reactions to methylated proteins/peptides.

BindingCapacity

Approximately 0.2 mg of acetylated histone / mL slurry.
Applications

Rapid isolation and purification of proteins/peptides with acetylated lysine residues from cell lysate or protease-digested mixtures.

Scientific Description

Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the acetylated species for further MS/MS identification.

Storage & Stability

Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Shake thoroughly before use.Expiration date is one year from date of shipping if properly stored.

Product Specific References
  1. 1.Science. 2009.325(5942): 834-840. doi:10.1126/science.1175371.
  2. 2.Cell. 2010.140(2): 257-267. doi:10.1016/j.cell.2009.12.031.
  3. 3.Mol. Cell.2010.39(2): 247-258. doi:10.1016/j.molcel.2010.07.006.
  4. 4.Proteomics. 2010.10(5): 1029-1039. doi:10.1002/pmic.200900602.
  5. 5.Plant Physiol. 2011.155(4): 1779-1790. doi:10.1104/pp.110.171595.
  6. 6.Euro. J. Cell Biol.2011.90(1-2): 128-135. doi:10.1016/j.ejcb.2010.09.004.
  7. 7.Mol. Cell. Proteomics.2012.11(11): 1510-1522. doi:10.1074/mcp.M112.017251.
  8. 8.Proc. Natl. Acad. Sci. U.S.A.2012.109(28): 11133-11138. doi:10.1073/pnas.1208669109.
  9. 9.J. Proteome Res. 2012.11(3): 1633-1643. doi:10.1021/pr2008384.
  10. 10.J. Lipid Res. 2012.53(9):1864-1876. doi:10.1194/jlr.M026567.
  11. 11.Cell. 2012.149(1): 214-231. doi:10.1016/j.cell.2012.02.013.
  12. 12.Cell.2012.150(3): 620-632.doi:10.1016/j.cell.2012.06.027.
  13. 13.Exp. Hematol. 2012.40(4): 342-355. doi:10.1016/j.exphem.2011.12.005.
  14. 14.PLoS ONE.2012.7(12): e50545. doi:10.1371/journal.pone.0050545.
  15. 15.PLoS Genet. 2012.8(9): e1002948. doi:10.1371/journal.pgen.1002948.
  16. 16.Euro. J. Cell Biol.2012.91(11-12): 950-960. doi:10.1016/j.ejcb.2012.07.001.
  17. 17.J. Proteomics.2013.79: 60-71. doi:10.1016/j.jprot.2012.12.001.
  18. 18.Biochem. Biophys. Res. Commun.2013.435(3): 403-407. doi:10.1016/j.bbrc.2013.04.101.
  19. 19.J. Biol. Chem.2013.288(36): 26209-26219. doi:10.1074/jbc.M113.483396.
  20. 20.J. Biol. Chem.2013.288(40): 29036-29045. doi:10.1074/jbc.M113.486753.
  21. 21.Mol. Microbiol. 2013.89(4): 660-675. doi:10.1111/mmi.12303.
  22. 22.Proc. Natl. Acad. Sci. U.S.A.2013.110(9): 3339-3344. doi:10.1073/pnas.1217632110.
  23. 23.Mol. Cell.2013.51(2): 265-272. doi:10.1016/j.molcel.2013.06.003.
  24. 24.Mol. Cell. Biol.2013.33(8): 1487-1502.doi:10.1128/MCB.00971-12.
  25. 25.PLoS ONE.2013.8(6): e64953. doi:10.1371/journal.pone.0064953.
  26. 26.Nature Methods.2013.10(7): 634-637. doi:10.1038/NMETH.2518.
  27. 27.Diabetes.2013.62(10): 3404–3417. doi:10.2337/db12-1650.
  28. 28.PLoS ONE. 2013.8(7): e67513. doi:10.1371/journal.pone.0067513.
  29. 29.J. Clin. Invest.2014.124(2):768-784. doi:10.1172/JCI69413.
  30. 30.PLoS ONE. 2014.9(2): e89283. doi:10.1371/journal.pone.0089283.
  31. 31.Mol. Syst. Biol.2014.10(11): 762.doi:10.15252/msb.20145227.
  32. 32.PLoS ONE. 2014.9(3):e91039. doi:10.1371/journal.pone.0091039.
  33. 33.Mol. Biosyst.,2015.11(3): 908-922 doi:10.1039/c4mb00490f.
  34. 34.Methods in Molecular Biology,2015.1295:275-292.doi:10.1007/978-1-4939-2550-6 21.
  35. 35.PloS one. 2015.10(10): e0140619. doi:10.1371/journal.pone.0140619.
  36. 36.J. Proteomics. 2015.128: 352-364. doi:10.1016/j.jprot.2015.08.015.
  37. 37.Plant Mitochondira: Methods and Protocols. 2015.107-121. doi:10.1007/978-1-4939-2639-8_7.
  38. 38.J Virol.2016 Feb 3. pii: JVI.03175-15.doi:10.1128/JVI.03175-15.
  39. 39.PLoS ONE. 2015.10(5): e0126242. doi:10.1371/journal.pone.0126242.
  40. 40.Nat Commun. 2015.6: 7726. doi:10.1038/ncomms8726.
  41. 41.Nat Biotechnol.2015.33(4): 415-423. doi:10.1038/nbt.3130.
  42. 42.EMBO Rep.2016.17(3): 455-469.doi:10.15252/embr.201541132.
  43. 43.Arch. Biochem. Biophys.2016.598: 1-10. doi:10.1016/j.abb.2016.03.025.
  44. 44. Cancers. 2016.8(3):37. doi:10.3390/cancers8030037.
  45. 45. mSystems.2016.1(3): e00005-16.doi:10.1128/mSystems.00005-16.
  46. 46. J Biol Chem. 2016.291(11):5484-5499. doi:10.1074/jbc.M115.707091.
  47. 47. Sci Rep. 2016.6:19722.doi:10.1038/srep19722.
  48. 48. JCI Insight. 2016.2(1):e84897.doi:10.1172/jci.insight.84897.
  49. 49. bioRxiv.2016. 057174. doi:http://dx.doi.org/10.1101/057174.
  50. 50.Archives of Biochemistry and Biophysics. 1-10. 2016. 598.
  51. 51.Molecular & Cellular Proteomics. 2016. doi: 10.1074/mcp.O116.065219
  52. 52.American Society for Microbiology. 2016. 1(3): 1-19. doi: 10.1128/mSystems.00005-16.
  53. 53.Cancers. 2016. 8(3): 1-13. doi: 10.3390/cancers8030037.
  54. 54.JCI Insight. 2016. 1(2): 1-14. doi: 10.1172/jci.insight.84897.
  55. 55.Universitat zu Koln. 2016. 1-169.
  56. 56.EMBO Press. 2016. 17(3): 455-469. doi: 10.15252/embr.201541132.
  57. 57.Nature Biotechnology. 2016. 34(11): 1198-1205. doi: 10.1038/nbt.3681.
  58. 58.Journal of Visualized Experiments. 2016. 108. doi: 10.3791/53563.
  59. 59.BioRxiv. 2016. doi: https://doi.org/10.1101/057174.
  60. 60.Journal of The American Society for Mass Spectrometry. 2016. 27 (11) 1758-1771. doi: 10.1007/s13361-016-1476-zg.
  61. 61. The Journal of Biological Chemistry. 2016. 291 (11) 5484-5499 doi: 10.1074/jbc.M115.707091.
  62. 62.Molecular & Cellular Proteomics. 2016. 15 (2): 493-505. doi: 10.1074/mcp.M115.049288.
  63. 63. PNAS. 2018. 115 (1): 210-215. doi:10.1073/pnas.1717519115
  64. 64.Methods Mol Biol. 2015;1305:107-21. doi:1007/978-1-4939-2639-8_7.
  65. 65. Mitochondrion. 2014 Nov;19 Pt B:252-60. doi:10.1016/j.mito.2014.03.004
  66. 66. Front. Cell. Infect. Microbiol. 2018. doi:10.3389/fcimb.2017.00537
  67. 67. Protein Acetylation. 2019. 1983:57-77. doi:10.1007/978-1-4939-9434-2_5
  68. 68. Molecular Metabolism. 2019. 25: 35-49. doi: 10.1016/j.molmet.2019.04.008
  69. 69. The Plant Journal. 2019. 99(1): 176-194. doi:10.1111/tpj.14315
  70. 70. Molecular Cell. 2019. 74(6): 1250-1263. doi: 10.1016/j.molcel.2019.04.009
  71. 71. Connective Tissue Research. 2019. doi: 10.1080/03008207.2019.1648443
  72. 72. Scientific Reports, 2020, 10(1), 2464.10.1038/s41598-020-59244-4
  73. 73. Mol. Systems Biol., 2020, 16(7), e9464.10.15252/msb.20209464
  74. 74. Journal of Mol. Cell Biol., 2020, 12(6), 424–437.10.1093/jmcb/mjz099
  75. 75. Mol. & Cell. Proteomics, 2020, 19(8), 1303–1309.10.1074/mcp.RA119.001897
  76. 76. Viruses, 2020, 12(9), 976.10.3390/v12090976

相关产品

matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY

intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15

goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP

novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg

novateinbio/SARS-Associated Coronavirus Spike Mosaic S ( C ) Recombinant ( SARS Mosaic S ( C ) )/500ug/PT_42025_500ug

matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB

intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24

goprolytix//10 ug, 100 ug/RTF-0300

最新签约

Liposoma

KAMIYA

Bioclone

intactgenomics

Abbexa

Emfret

Tribioscience

goprolytix

技术文章

ProChimia/HS(CH2)mCOOH/FT 002m101/1 g

Alomone经销商/代理商 Alomone蛋白质

prospec mSCF

【供应 GelRed核酸染料 （Biotium 41003）】价格_厂家 苏州蚂蚁淘

PDMS Device Fabrication and Surface Modification | Protocol

prospec HOR 250

上海正品NSJ Bioreagents生物试剂1 NSJ Bioreagents细胞生物学

杭州Enzymatics inc批发，产品货源 Enzymatics T4连接酶

新闻动态

苏州Enzymatics型号参数规格 Enzymatics超纯poly酶

19413561 GAMMASECRETASE INHIBITOR X 1MG

合肥中国Abbexa官网，Abbexa Ltd价格更新 abbexa授权代理

Abbexa中国采购 abbexa纯化蛋白

RhodamineDHPE美国/Biotium询价/索取资料——苏州蚂蚁淘

武汉美国Polysciences公司 分析试剂Polysciences

广州Anatrace型号参数规格 Anatrace膜蛋白提取与纯化

Genovis 特约代理苏州蚂蚁淘实验试剂有限公司

行业前沿

正品Pfenex生物试剂 重组白喉杆菌CRM197,为什么这么便宜?

PfenexX中国代理价格，厂家 SEA133鲑鱼纤维蛋白原,求推荐!

Pfenex中国采购 三文鱼凝血酶/鲑鱼凝血酶 Salmon Thrombin,是正品吗?

Polysciences聚乙烯亚胺 Polyethylenimine,好用吗?

PolysciencesX中国代理价格，厂家 Polysciences24765,哪里有卖?

Polysciences代理，产品目录 Polysciences聚合物,是正品吗?

Sea Run Holding产品促销 重组白喉杆菌CRM197,为什么这么便宜?

生命科学Pfenex价格优惠 Sea Run Holding代理，产品目录,有优惠活动吗?

最热产品

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003

Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003

Qubit™ dsDNA BR Assay Kit Q32853现货

Parafilm M封口膜/PM996/4x125/整箱

Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销

LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货

GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g

Leica徕卡819一次性窄刀片【50片/盒，10盒/条】/14035838925

推荐新闻

RA Biosources报价单 rabios代理

Lampire型号参数规格 Lampire公司

品牌试剂实验Medica Lisotopes代理 Medica Lisotopes产品

进口试剂QoolAbs直采 Qoolabs噬菌体筛选

进口Molecular Research Center品牌清关代理 MRC一步法TRIzol RNA抽提

授权代理商，正品 Spherefluidics授权

Ursabioscience美元价/进货价 Ursa BioScience产品

Wards Science抗体，试剂，血清，细胞 wardsci授权

新闻动态
行业前沿
技术文章
最新产品