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Product Description | The rabbit anti-acetyl lysine antibody (anti-AcK) is developed using acetylated KLH as the immunogen. It is affinity purified with acetyl lysine agarose, followed by conjugation with horseradish peroxidase(HRP). |  Western blot analysis of acetylated histone from TSA-treated (A) and non-treated (B) melanoma cells using ICP0381. | |
Species | Rabbit | ||
Formulation | 250 µg/mL in stabilizing buffer with 50% glycerol | ||
Immunogen | Acetylated KLH conjugates | ||
Purification | Affinity chromatography with acetyl-lysine agarose | ||
Conjugation | Reductive amination; HRP:Ab molar ratio =10:1 | ||
Specificity | The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA and acetylated MBP. No cross-reaction to the non-acetylated proteins. | ||
Applications | For direct immunofluorescence experiments | ||
Scientific Description | DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating the lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g. transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. | ||
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