产品资料

Addgene/pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3/1unit/112005-AAV5

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	112005	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV5	112005-AAV5	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV9	112005-AAV9	Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pAAV
(Search Vector Database)
Backbone sizew/o insert(bp)4603
Total vector size (bp)6787
Vector type
Mammalian Expression, AAV

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
NEB Stable
Copy number
High Copy

Gene/Insert

Gene/Insert name
axon-GCaMP6s-P2A-mRuby3
Alt name
GAP43-GCaMP6s-P2A-mRuby3
Species
Synthetic
Insert Size (bp)
2184
GenBank ID
MH282429
PromoterhSynapsin1
Tag/ Fusion Protein
- GAP43 palmitoylation domain (N terminal on insert)

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteHindIII(not destroyed)
5′ sequencing primerGAAGGCGCGCTGACGTCACTCGCCG
3′ sequencing primerCAAATTTTGTAATCCAGAGGTTGATT

Resource Information

Supplemental Documents
- pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3.gbk
Terms and Licenses
- UBMTA
Industry Terms
- Not Available to Industry

Depositor Comments

Addgene NGS results found minor differences in the second ITR compared to the depositor-provided sequence. The depositing lab has confirmed that this does not impair AAV production

Information for AAV5 (Catalog # 112005-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA.

Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GenemRuby3

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 112005-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA.

Delivery

Volume100 µL
Titer≥ 1×10¹³ vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GenemRuby3

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

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Addgene/pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3/1unit/112005-AAV5

Ordering

Backbone

Growth in Bacteria

Gene/Insert

Cloning Information

Resource Information

Depositor Comments

Information for AAV5 (Catalog # 112005-AAV5)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control

Information for AAV9 (Catalog # 112005-AAV9)(Back to top)

Purpose

Delivery

Viral Production & Use

Biosafety

Resource Information

Viral Quality Control