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Addgene/pMIEG3-NFAT2/1unit/40351

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ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid40351Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIEG3
    (Search Vector Database)
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFAT2
  • Alt name
    nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
  • Species
    M. musculus (mouse)
  • GenBank ID
    NG_029226.1
  • Entrez Gene
    NFATC1(a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Tag/ Fusion Protein
    • IRES eGFP (C terminal on backbone)

Cloning Information

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteXhoI(destroyed during cloning)
  • 3′ cloning siteXhoI(destroyed during cloning)
  • 5′ sequencing primerpLXSN_5
  • 3′ sequencing primerIRES-R2 (gacggcaatatggtggaaa)
    • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
    • Ancillary Agreement for Plasmids Containing FP Materials
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The NFAT2-expressing retroviruses were constructed by inserting blunt-ended NFAT2 cDNA from pSH160C into the retroviral vector pMIEG3 via the blunt-ended XhoI site.

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