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Addgene/pAAV.CMV.PI.EGFP.WPRE.bGH/1unit/105530-AAV9

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid105530Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart
AAV1105530-AAV1Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV2105530-AAV2Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV5105530-AAV5Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV8105530-AAV8Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV9105530-AAV9Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart
AAV rh10 (similar to AAV10)105530-rh10Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information
$380
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
    (Search Vector Database)
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP
  • PromoterCMV

Cloning Information

  • Cloning methodUnknown
  • 5′ sequencing primerCMV-F
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
    • pAAV.CMV.PI.EGFP.WPRE.bGH (p0101).gb
  • Terms and Licenses
    • UBMTA
    • Ancillary Agreement for Plasmids Containing FP Materials
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
    • 1 Reference

Depositor Comments

Penn Vector Core number p0101

Information for AAV1 (Catalog # 105530-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV1
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 105530-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 7×10¹² vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV2
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 105530-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 7×10¹² vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
  • BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
  • SerotypeAAV5
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 105530-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV8
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 105530-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAV9
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV rh10 (similar to AAV10) (Catalog # 105530-rh10)(Back to top)

Purpose

Ready-to-use AAV rh10 (similar to AAV10) particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.

CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume100 µL
  • Titer≥ 1×10¹³ vg/mL
  • Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • StorageStore at -80℃. Thaw just before use and keep on ice.
  • ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAVrh10 cap gene
  • BufferPBS + 0.001% Pluronic F-68
  • SerotypeAAVrh10
  • PurificationIodixanol gradient ultracentrifugation
  • Reporter GeneEGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

  • Terms and Licenses
    • Ancillary Agreement for Penn Vectors
    • Terms of Use for Viral Vectors
  • Industry Terms
    • Not Available to Industry

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

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