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Addgene/pWZL Neo Myr Flag CDKN1B/1unit/20420

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ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid20420Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWZL-Neo-Myr-Flag-DEST
    (Search Vector Database)
  • Backbone manufacturer
    William Hahn Lab (available at Addgene, #15300)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3 cells to prevent recombination.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDKN1B
  • Alt name
    cyclin-dependent kinase inhibitor 1B
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDKN1B(a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
  • Tags/ Fusion Proteins
    • Myr (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning methodGateway Cloning
  • 5′ sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)
  • 3′ sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    3-D8
  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry

Depositor Comments

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations.Please see the following link for plasmids from this article that are not part of the kinase libraryhttp://www.addgene.org/pubmed/17574021

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