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Addgene/pTU2-A-RFP (p15A origin)/1unit/74090

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ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid74090Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p15A origin and chloramphenicol resistance
    (Search Vector Database)
  • Backbone manufacturer
    Terrence Lai (this study)
  • Backbone sizew/o insert(bp)2024
  • Total vector size (bp)3286
  • Modifications to backbone
    Modifications between XbaI and PstI from original pSB1C3 plasmid. CAT gene - C435G (nucleotide) - silent mutagenesis to remove BsmBI site
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    10 ml LB culture - use 2X P1, P2 and N3 buffer for mini-prep purification and concentrate elution fraction (~10-20 ng/ul in 50 ul total volume of water) until 50 ng/ul for Golden Gate
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
  • Species
    Synthetic
  • PromoterR0010 Const. Promoter controlling RFP

Cloning Information

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteBglII(destroyed during cloning)
  • 3′ cloning siteSpeI(destroyed during cloning)
  • 5′ sequencing primerCTGACGTCTAAGAAACCATT
  • 3′ sequencing primerAACCGTATTACCGCCTTTGA
    • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
    • Takara Bio Limited Use Label License (formerly Clontech)
  • Industry Terms
    • Not Available to Industry
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