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MACS/StraightFrom Buffy Coat CD14 MicroBead Kit, human/130-114-976/

Capacity:for1buffycoat
Components:
  • 4mLStraightFromBuffyCoatCD14MicroBeads
  • 12×WholeBloodColumns
  • WholeBloodColumnElutionBuffer

Overview

TheStraightFrom™BuffyCoatCD14MicroBeadKithasbeendevelopedfortherapidpositiveselectionofCD14+cellsdirectlyfromonebuffycoatusingtheMultiMACS™Cell24SeparatorPlusforsemi-automatedseparationoraMACS®Separatorformanualseparation.Nosamplepreparationisrequired,includingdensitygrADIentcentrifugation,erythrocytelysis,washsteps,orcellcount.WholeBloodColumnsandWholeBloodColumnElutionBufferareincludedinthekittoallowdirectprocessingofanentirebuffycoat.

Details

Backgroundinformation

TheCD14antigenbelongstotheLPSreceptorcomplex.Itisstronglyexpressedonmostmonocytesandmacrophagesandweaklyonneutrophils.

Detailedseparationprocedure

OneentirebuffycoatislabeledwithStraightFrom™BuffyCoatCD14MicroBeadsandloadedontoWholeBloodColumns,whichareplacedinthemagneticfieldofaMultiMACS™Cell24SeparatorPlusoraMACS®Separator.Afterremovingthecolumnfromthemagneticfield,themagneticallyretainedCD14+cellscanbeelutedasthepositivelyselectedcellfraction.

Downstreamapplications

ThepurifiedCD14+cellsarewell-suitedforfurtherflowcytometric,functional,ormolecularanalysis.
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