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Diapharma/Technoclone Anti-PAI-1 Monoclonal Antibody 1PAI/TC21163/Vial/500µg

Description:

TechnocloneAnti-PAI-1MonoclonalAntibody1PAI

IgG2;halfmaximalbindingtocoatedPAI-1at1µg/ml;reactionwithactivePAI-1,latentPAI-1andt-PA-PAI-1complexes;nocrossreactionwithPAI-2orPAI-3;noeffectonthefunctionalactivityofPAI-1.ApplicationsincludeimmunodepletionofPAI-1fromhumanplasmainELISAtests

Specificity

BindstoactivePAI-1,latentPAI-1andt-PA-PAI-1complexes.NobindingtoPAI-2orPAI-3.NoeffectonthefunctionalactivityofPAI-1.

Derivation

HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedPAI-1fromthehumanmelanomacelllineMJZJaccordingtothemethodofWagneretal.(1).

Antibodyclass

IgG2b

Purification

TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.

Handlingandstorage:

Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazide.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.

Literature:

1)O.F.Wagner,M.Vetterlein,andB.R.Binder:Purificationofanactiveplasminogenactivatorinhibitorimmunologicallyrelatedtotheendothelialtypeplasminogenactivatorinhibitorfromtheconditionedmediaofahumanmelanomacellline.J.Biol.Chem.261:14474,1986.Tech

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