Reactswithurokinaseanditscomplexes;noreactionwithotherplasmaproteins. CanbeusedasprecipitatingantibodyinRIA(2,3),asantibodyinanELISA(3)orforimmunoaffinitypurificationofurokinase(4).
Highmolecularweighturokinase
Rabbit
TheantibodyispurifiedfromrabbitserumbyammoniumsulphateprecipitationfollowedbyDEAEion-exchangechromatography.
(1)Reactswithhighandlowmolecularweighturokinaseaswellwithscu-PA(urine,tissuecultureandrecombinant);alsoreactswithu-PAinhibitorcomplexes.
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof1mgandshouldbereconstitutedwith1mLdistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
1)K.Huber,J.Kirchheimer,B.R.Binder:Rapidisolationofhighmolecularweighturokinasefromnativehumanurine.Thromb.Haemost.47:197–202,1982.
2)K.Huber,J.Kirchheimer,B.R.Binder:Characterizationofaspecificanti-humanurokinaseantibody:developmentofasensitivecompetitionrADIoimmunoassayforurokinaseantigen.J.Lab.Clin.Med.103:684–694,1984.
3)J.Wojta,B.R.Binder,K.Huber,R.L.Hoover:Evaluationoffibrinolyticcapacityinplasmaduringthrombolytictherapywithsingle(scu-PA)ortwochainurokinasetypeplasminogenactivator(tcu-PA)byacombinedassaysystemforurokinasetypeplasminogenactivatorantigenandfunction.Thromb.Haemost.61:289-293,1989.
4)B.Grasl,M.Jörg,B.R.Binder:Isolationofaplasminogenactivatorfromhumanplasmabyaffinitychromatographyonanti-urokinase-Sepharose.Partialcharacterizationoftheenzyme.In:Progressinfibrinolysis,Vol.6,ChurchillLivingstone,Edinburgh,London,Melbourne,NewYork,pp.50-53,1983.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn