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Glen research/dG-CE Phosphoramidite/1kit/10-1020-10

Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Suggested Laboratory Practices to Prevent Precipitation of dG Phosphoramidite

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA


Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
DiluentAnhydrous Acetonitrile
StorageControlled room temperature or lower, dry
StabilityNormal

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
10-1020-020.25 g.25grams2.988651.632.2523.4517.24.3
10-1020-050.5 g.5grams5.9518511169.3850.45379.25
10-1020-101.0 g1grams11.91383.67230.2143.88104.6476.7319.18
10-1020-202.0 g2grams23.81780.33468.2292.63212.82156.0739.02
10-1020-404.0 g4grams47.621574944.4590.25429.27314.878.7

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
10-1020-C20.25 g.25grams4.440.0785.653.538.915.35
10-1020-C50.5 g.5grams8.880.07174.410979.2810.9
10-1020-1C1.0 g1grams17.770.07352.2220.13160.0922.01
10-1020-2C2.0 g2grams35.540.07707.6442.25321.6444.23


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