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Details
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Technical Documents

Safety Data Sheet

Description

An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl- phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: ultraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours in Ammonium Hydroxide at room temperature.
Specifications
StorageRefrigerated storage, maximum of 2-8°C, dry

Related Products

Pac-dA-CE Phosphoramidite
Pac-dA-CE PhosphoramiditeCatalog #: 10-1601
iPr-Pac-dG-CPG
iPr-Pac-dG-CPGCatalog #: 20-2621
Pac-A-CE Phosphoramidite
Pac-A-CE PhosphoramiditeCatalog #: 10-3000
Ac-dC-CE Phosphoramidite
Ac-dC-CE PhosphoramiditeCatalog #: 10-1015
5% Phenoxyacetic anhydride in THF/Pyridine
5% Phenoxyacetic anhydride in THF/PyridineCatalog #: 40-4210
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