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Glen research/CCA Trimer Phosphoramidite/1kit/13-1110-90

Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Glen Report 24.23: Antisense Trimer Phosphoramidites - A New Method for Mutagenesis Spanning Entire Genes

Glen Report 25.12: Antisense Trimer Phosphoramidites - Update

Glen Report 20.111: Technical Brief - Phage Display, Artificial Antibodies and Trimer Phosphoramidites

Glen Report 32.15: Application Note — Trimer Phosphoramidites

Description

All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys). For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact [email protected] for a quotation and projected delivery date.

Details

Usage

  • Coupling: 15 minute coupling time recommended.
  • Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Specifications
DiluentAnhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
StorageFreezer storage, -10 to -30°C, dry
Stabilityn/a

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
13-1110-90100 µmol.186grams120127.55.4541
13-1110-9550 µmol.093grams0.53.3321.250.910.670.17

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
13-1110-90100 µmol.186grams1.50.0723.614.7510.731.48
13-1110-9550 µmol.093grams0.750.078.65.383.910.54

Related Products

Trimer Phosphoramidite Mix 2
Trimer Phosphoramidite Mix 2Catalog #: 13-1992
Trimer Phosphoramidite Mix 1
Trimer Phosphoramidite Mix 1Catalog #: 13-1991
ACC Trimer Phosphoramidite
ACC Trimer PhosphoramiditeCatalog #: 13-1011
AGA Trimer Phosphoramidite
AGA Trimer PhosphoramiditeCatalog #: 13-1020
CGG Trimer Phosphoramidite
CGG Trimer PhosphoramiditeCatalog #: 13-1122
GAT Trimer Phosphoramidite
GAT Trimer PhosphoramiditeCatalog #: 13-1203
GCA Trimer Phosphoramidite
GCA Trimer PhosphoramiditeCatalog #: 13-1210
GCG Trimer Phosphoramidite
GCG Trimer PhosphoramiditeCatalog #: 13-1212
GTA Trimer Phosphoramidite
GTA Trimer PhosphoramiditeCatalog #: 13-1230
TTT Trimer Phosphoramidite
TTT Trimer PhosphoramiditeCatalog #: 13-1333
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