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Glen research/CAG Trimer Phosphoramidite/1kit/13-1102-90

Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Glen Report 16.25: Trimer (Codon) Phosphoramidites Simplify Library Preparation

Glen Report 18.12: Trimer Phosphoramidites – Tools for fine-tuning protein function

Glen Report 25.12: Antisense Trimer Phosphoramidites - Update

Glen Report 20.111: Technical Brief - Phage Display, Artificial Antibodies and Trimer Phosphoramidites

Glen Report 32.15: Application Note — Trimer Phosphoramidites


Description

All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are available: 13-1991-xx, for incorporating all 20 amino acid codons equally into a sequence and 13-1992-xx, for incorporating 19 amino acid codons (-Cys). For a custom trimer mix of a particular subset of codons or a trimer mix that represents a set of trimers that is biased toward a particular codon or codons, please contact [email protected] for a quotation and projected delivery date.

Details

Usage

  • Coupling: 15 minute coupling time recommended.
  • Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Specifications
DiluentAnhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
StorageFreezer storage, -10 to -30�C, dry
Stability2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
13-1102-90100 µmol.187grams120127.55.4541
13-1102-9550 µmol.093grams0.53.3321.250.910.670.17

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
13-1102-90100 µmol.187grams1.50.0723.614.7510.731.48
13-1102-9550 µmol.093grams0.750.078.65.383.910.54


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