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WGS Ligase is optimized for ligation following WGS Fragmentation. It catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex RNA, RNA, or DNA/RNA hydrid (1).
Source of ProteinA recombinant E.coli strain carrying the cloned T4 DNA Ligase gene
Unit Definition1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C.
Molecular Weight55,292 Daltons
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