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Epigentek/EpiQuik Long Taq DNA Polymerase/R120123/2500 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Epigentek/EpiQuik Long Taq DNA Polymerase/R120123/2500 Units
ReagentType:PCREnzyme
100%Guarantee:6months
ProductOverview

Description
EpiQuikLongTaqDNAPolymeraseisthemixtureoftwoDNApolymerasesdesignedforlongPCR.ThemixtureallowstoamplifygenomicDNAfragmentsupto20kbandviralDNAfragmentsupto40kb.

Concentration 
5unit/µl(oneunitoftheenzymecatalyzestheincorporationof10nanomolesofdeoxyribonucleotidesintoapolynucleotidefractionin30minat70°C).

Source 
RecombinantE.colistrainwithclonedgeneencodingThermusaquaticusDNApolymerase.

Composition

  • EpiQuikLongTaqDNAPolymeraseinStorageBuffer:20mMTris-HCl(pH8.0),1mMDTT,0.1mMEDTA,100mMKCl,0.5%NonidetP40,0.5%Tween20and50%glycerol 
  • 10xReactionBuffer:100mMTris-HCl(pH8.8at25°C),500mMKCl,0.8%NonidetP40
  • 10xReactionBufferwith(NH4)2SO4:750mMTris-HCl(pH8.8at25°C),200mM(NH4)2SO4,0.1%Tween20
  • 25mMMgCl2Solution

Applications

  • StandardandlongPCR
  • PCRwithdifficulttemplates


StorageConditions
Storeat-20°C.Guaranteedstablefor12monthswhenproperlystored.

Fig.1. Amplificationof5,3KbtargetDNAusingEpiQuikLongTaqDNAPolymerase.

1-2–EpiQuikLongTaqDNAPolymerase

M–EpiQuik1KbDNALadderPlus


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