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Epigentek/EpiQuik Dnmt3A Assay Kit/P-3012-3/96 assays

InputType:NuclearExtracts,PurifiedEnzyme
ResearchArea:DNAMethylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™Dnmt3AAssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofDnmt3Aattremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDnmt3Aassayexperiment.TheEpiQuik™Dnmt3AAssayKitissuitableformeasuringDnmtamountsquantitativelyfromfreshtissueandculturedcellsofhumanandmouse.Thekithasthefollowingadvantages:

  • Thefastestprocedure,whichcanbefinishedwithin3hours.
  • Innovativecolorimetricassay,whichquantitativelymeasureDnmt3Aamountwithoutthe needforelectrophoresis.
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™Dnmt3AAssayKitisdesignedformeasuringtotalDnmt3Aamountfromtissuesorcells.Inanassaywiththiskit,theuniqueDnmtaffinitysubstrateisstablycoatedonthestripwell.ThesampleisaddedintothewellandDnmt3Acontainedinthesamplebindstothesubstrate.TheboundDnmt3Acanberecognizedwitha specificDnmt3AantibodyandcolorimetricallyquantifiedthroughanELISA-likereaction.TheamountofDnmt3Aisproportionaltotheintensityofcolordevelopment.

 
Fig.1.SchematicprocedureforusingtheEpiQuikDnmt3AAssayKit.

Fig.2. NuclearextractswerepreparedfromMCF-7cellsusingtheEpiQuikNuclearExtractionKitandtotalamountofDnmtwasmeasured.

ProductComponents

DA1(10XWashBuffer)
DA2(AssayBuffer)
DA3(Dnmt3AStandard,20µg/ml)*
DA4(CaptureAntibody,100µg/ml)*
DA5(DetectionAntibody,200µg/ml)*
DA6(DevelopingSolution)
DA7(StopSolution)
BB(BlockingBuffer)
8-WellAssayStrips(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.

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