Epigentek/EpiQuik Methylated DNA Immunoprecipitation Kit/P201948/48 reactions

Pleaseseethe MethylampDNACaptureKit ifyouareworkingwithpurifiedDNAasthestartingmaterial. 


The EpiQuik™MethylatedDNAImmunoprecipitation(MeDIP)Kitisacompletesetofessentialcomponentswhichusesaproprietaryanduniqueprocedure/compositiontoenrichmethylatedDNAfromcells.Intheassay,anantibodyspecifictomethylcytosineisusedtoimmunoprecipitatemethylatedgenomicDNA.TheimmunoprecipitatedmethylatedfractionscanbethenusedforastandardDNAdetection.ThekitissuitableforcombiningthespecificityofmethylatedDNAimmunoprecipitationwithqualitativeandquantitativePCR,andsouthernblotaswellasDNAmicroarray.Thekithasthefollowingadvantages:

• DirectlyimmunoprecipitatethemethylatedfractionsofDNAfromcelllysates.
• HighlyefficientenrichmentofmethylatedDNA:>95%.
• Stripmicroplateformatallowsmanualorhighthroughput.
• ConvenientlyincludescolumnsforDNApurificationinordertosavetimeandreducelabor.
• CompatiblewithallDNAamplification-basedapproaches.
• Achievesveryreliableandconsistentassayconditions.

Principle&Procedure
TheEpiQuik™MethylatedDNAImmunoprecipitation(MeDIP)KitcontainsallreagentsrequiredforcarryingoutasuccessfulmethylatedDNAimmunoprecipitationdirectlyfrommammaliancells.Particularly,thiskitincludesaChIP-grade5-methylcytosineantibodyandanegativecontrolnormalmouseIgG.DNAinthecellsisextracted,sheared,andaddedintothemicrowellimmobilizedwiththeantibody.DNAisreleasedfromtheantibody-DNAcomplex,andpurifiedthroughthespecificallydesignedFast-SpinColumn.ElutedDNAcanbeusedforvariousdown-streamapplications.

CP1(WashBuffer)
CP2(AntibodyBuffer)
CP3A(Pre-LysisBuffer)
CP3B(LysisBuffer)
CP4(ChIPDilutionBuffer)
CP5(DNAReleaseBuffer)
CP6(ReverseBuffer)
CP7(BindingBuffer)
CP8(ElutionBuffer)
NormalMouseIgG(1mg/ml)*
Anti-5-Methylcytosine(1mg/ml)*
ProteinaseK(10mg/ml)*
8-WellAssayStrips(withFrame)
8-WellStripCaps
F-SpinColumn
F-CollectionTube
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.