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| InputType: | Tissues |
| ResearchArea: | RNAMethylation |
| TargetApplication: | SampleIsolation |
| VesselFormat: | Columns/Tubes |
| 100%Guarantee: | 6months |
TheEpiQuik™ TotalRNAIsolationKit(Tissue) providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromtissuesamples.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspeciesbasestobindtotheglassfibermatrixofthespincolumnwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureRNAiselutedwithREBufferwithoutphenolextractionoralcoholprecipitation.RNApurifiedwithTheTotalRNAIsolationKit(Tissue)issuitableforavarietyofroutineapplicationsincludingRT-PCR,cDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNASelection.Theentireprocedurecanbecompletedwithin25-40minutes. Thekithasthefollowingfeaturesandadvantages:
Principle&Procedure
ThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromtissue.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications.
StartingMaterials
Theamountofstartingmaterialscanbeupto30mgoftissue,orupto25mgofparaffin-embeddedtissue.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype.
RR(LysisBuffer)
W1(WashBuffer-1)
W2(WashBuffer-2)*
RE(ElutionBuffer)
T-Column
CollectionTubes
*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.