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Epigentek/EpiQuik HAT Activity/Inhibition Assay Kit/P400396/96 assays
InputType:NuclearExtracts,PurifiedEnzyme
ResearchArea:HistoneAcetylation
TargetApplication:ActivityMeasurement
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™HATActivity/InhibitionAssayKitisaready-to-usesetofessentialcomponentsneededtomeasurehistoneacetyltransferaseactivity/inhibitionsafelyandquickly.Itissuitableformeasuringhistoneacetyltransferaseactivity/inhibitionfromabroadrangeofspeciesincludingmammaliancells/tissues,plants,andbacteria.Thekithasthefollowingadvantages:

  • Veryquickprocedure,whichcanbecompletedwithinonly3hours.
  • Innovativecolorimetricassaywithoutradioactivity,extraction,andchromatography.
  • DirectmeasurementofHATactivityandinhibitionbyquantifyingtheamountofacetylatedhistonesubstrate,therebyavoidingthefalseinhibitoryeffectonHATs.
  • Stripmicroplateformatmakestheassayflexibleinbothmanualandhighthroughputanalysisformats.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™HATActivity/InhibitionAssayKitisdesignedformeasuringtotalhistoneaceyltransferaseactivityorinhibition.Inanassaywiththiskit,theuniquehistonesubstrateisstablycapturedonthestripwells.ActiveHATsbindtoandacetylatehistonesubstrate.Theacetylatedsubstratecanberecognizedwithahighaffinityanti-acetylatedhistoneantibody.Theratiooramountoftheacetylatedhistone,whichisdirectlyproportionaltoHATenzymeactivity,canthen be colorimetricallyquantifiedthroughanELISA-likereaction.

 
Fig.1.SchematicprocedureforusingtheEpiQuikHATActivity/InhibitionAssayKit.

Fig.2. IllustratedstandardcurvegeneratedwithHATassaystandard.

Fig.3. NuclearproteinswerepreparedfromdifferentcelllinesandHATactivitywasmeasuredusingtheEpiQuik™HATActivity/InhibitionAssayKit.

ProductComponents

HT1(10XWashBuffer)
HT2(HATSubstrate,20µg/ml)*
HT3(HATStandard,20µg/ml)*
HT4(AcetylCo-A,30mM)*
HT5(HATAssayBuffer)
HT6(CaptureAntibody,100µg/ml)*
HT7(DetectionAntibody,200µg/ml)*
HT8(DevelopingSolution)
HT9(StopSolution)
8-WellAssayStrip(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.


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