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Epigentek/EpiQuik Global Histone H3 Phosphorylation (Ser28) Assay Kit (Colorimetric)/P700496/96 as188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Epigentek/EpiQuik Global Histone H3 Phosphorylation (Ser28) Assay Kit (Colorimetric)/P700496/96 as
InputType:CellExtracts,HistoneExtracts,UnisolatedSamples
ResearchArea:Phosphorylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™GlobalHistoneH3Phosphorylation(Ser28)AssayKit(Colorimetric)isaconvenientsetoftoolsthatallowstheexperimentertomeasureglobalphosphohistoneH3(ser28).Thekitisready-to-useandprovidesalltheessentialcomponentsneededforspecificallymeasuringglobalhistoneH3phophorylationatser28usingavarietyofmammaliancells(human,mouse,etc.)includingfreshandfrozentissues,andculturedadherentandsuspensioncells.Thekithasthefollowingadvantages:

  • Quickandefficientprocedure,whichcanbefinishedwithin3hours.
  • Innovativecolorimetricassaywithouttheneedforradioactivity,electrophoresis,orchromatography.
  • SpecificallycapturesphosphohistoneH3(ser28)withthedetectionlimitaslowas2ng/well.
  • ThecontrolisconvenientlyincludedforthequantificationofphosphorylatedhistoneH3(ser28).
  • Stripmicroplateformatmakestheassayflexible:manualorhighthroughput.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™GlobalHistoneH3Phosphorylation(Ser28)AssayKit(Colorimetric)isdesignedformeasuringglobalhistoneH3phosphorylationatser28.Inanassaywiththiskit,thephosphorylatedhistoneH3atser28iscapturedtothestripwellscoatedwithananti-phosphohistoneH3(ser28)antibody.ThecapturedphosphohistoneH3(ser28)canthenbedetectedwithalabeleddetectionantibody,followedbyacolordevelopmentreagent.TheratioofphosphohistoneH3(ser28)isproportionaltotheintensityofabsorbance.TheabsoluteamountofphosphohistoneH3(ser28)canbequantitatedbycomparingtothestandardcontrol.

 
Fig.1.SchematicprocedureforusingtheEpiQuikGlobalHistoneH3Phosphorylation(Ser28)AssayKit(Colorimetric).

ProductComponents

C1(10XWashBuffer)
C2(AntibodyBuffer)
C3(DetectionAntibody,1mg/ml)*
C4(ColorDeveloper)*
C5(StopSolution)
StandardControl(100µg/ml)*
8-WellSampleStrips(withFrame)
8-WellStandardControlStrips
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.


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