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| ExpressedIn: | E.Coli |
| ProteinSpecies: | Human |
| ResearchArea: | HistoneSumoylation |
| 100%Guarantee: | 6months |
Description
SUMO-2modifiedwithbiotinviaprimaryaminecouplingresultinginmodificationoflysineresiduesaswellastheN-terminus.AlthoughhavingafullyfunctionalC-terminus,lysinemodificationmaylimittheabilityofthisreagenttopropagatepoly-SUMOchains.BiotinylatedSUMO-2canbedetectedusingavidin-linkedreagentsforhigherefficiencyandsensitivitythanwithantibodies.
MolecularWeight(kDa)
10.6kDa
Formulation
50mMHEPESpH8.0,150mMNaCl,1mMDTT,10%glycerol
Source
ExpressedinE.coliexpressionsystem
Size
50µg
StorageTemp&Conditions
Storeat-80°C.Avoidmultiplefreeze/thawcycles.
PurificationNotes
Purity:>95%bySDS-PAGE
ApplicationNotes
Enzyme-linkedImmunoabsorbentAssay;WesternBlot(Recombinantprotein;ProteinArray;;Bestusewithinthreemonthsfromthedateofreceiptofthisprotein.