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NEB/Q5® Hot Start High-Fidelity 2X Master Mix/M0494S/100 reactions (50 μl vol)188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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NEB/Q5® Hot Start High-Fidelity 2X Master Mix/M0494S/100 reactions (50 μl vol)

Description:

  
TheQ5®HotStartHigh-Fidelity2XMasterMixfeaturesahigh-fidelity,Thermostable,hotstartDNApolymerasewith3´→5´exonucleaseactivity,fusedtoaprocessivity-enhancingSso7ddomaintosupportrobustDNAamplification.Theadditionofanaptamer-basedinhibitorallowsroomtemperaturereactionsetup.Withanerrorrate~280-foldlowerthanthatofTaqDNAPolymerase,Q5HotStartHigh-Fidelity2XMasterMixisidealforcloningandcanbeusedforlongordifficultamplicons.Theconvenientmastermixformulationissuppliedata2Xconcentration.ThemixcontainsdNTPs,Mg++andaproprietarybroad-usebufferrequiringonlytheadditionofprimersandDNAtemplateforrobustamplificationregardlessofGCcontent.Whenusedattherecommended1Xfinalconcentration,theQ5HotStartHigh-FidelityMasterMixcontains2mMMg++.Q5HotStartHigh-Fidelity2XMasterMixisunliketypical,lowerfidelityPCRenzymes.Todeterminetheoptimalannealingtemperaturesforagivensetofprimers,useoftheNEBTmCalculatorishighlyrecommended.

Q5-HighFidelity2XMasterMixformatsallowrobustamplificationofabroadrangeoftargetswithasingleformulation. 

M0494
AmplificationofavarietyofhumangenomicampliconsfromlowtohighGCcontentusingQ5HotStartHigh-Fidelity2XMasterMix.Allreactionsweresetupatroomtemperature,conductedusing30cyclesofamplificationandvisualizedbymicrofluidicLabChip®analysis.
  

ProductSource

AnE.colistrainthatcarriestheQ5High-FidelityDNAPolymerasegene.

Notes:

Aprecipitate(mostnoticeableafterthefirst1–2freeze/thawcycles)isnotuncommon.Toensureoptimalperformance,themastermixshouldbethawedandresUSPendedpriortouse.StABIlitytestingusingupto15freeze/thawcycleshasshownnonegativeeffectonmastermixperformance.ProductspecificationsforindividualcomponentsintheQ5HotStartHigh-Fidelity2XMasterMixareavailableseparately.
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